Webinar: Validating Single-Cell RNA-seq Results with Milo and RNAscope


Now Available On-Demand!
Validating Single-Cell RNA-seq Results with Milo and RNAscope


This re-broadcast webinar with a live Q&A will cover the basics of the Milo Single-Cell Western blot (SCW) and RNAscope single-molecule fluorescence RNA in situ hybridization (smFISH) technologies and present data from an ongoing study in the laboratory of Dr. Brian Hermann at the University of Texas at San Antonio where both platforms are being used to validate single-cell RNA-seq data.

Learning Objectives:
  • Understand how Milo Single-Cell Westerns can provide protein-level validation of heterogeneity among individual cells uncovered by single-cell RNA-seq (scRNA-seq). Learn more.
  • Understand how RNAscope smFISH can be used to validate scRNA-seq data at the mRNA level and enable spatial remapping to determine localization of cells with particular phenotypes. Learn more.

Jennie Rohrer, Dr. TuAnh Dang and Sun Boi from Bio-Techne will be on the call afterwards to answer questions about Milo and RNAscope.

SPEAKERS

Brian Hermann

Dr. Brian Hermann
Associate Professor and Genomics Core Director
University of Texas at San Antonio

Dr. Hermann studies spermatogonial stem cells (SSCs), which are adult stem cells responsible for life-long sperm production in the testes of mammals. Single-cell RNA-seq studies from his laboratory identified substantial gene expression heterogeneity among undifferentiated spermatogonia (which include both SSCs and the progenitor spermatogonia that they produce) at the mRNA level. Results from ongoing validation studies in his laboratory will be presented. Dr. Hermann has used Milo SCW to confirm gene expression heterogeneity at the protein level among individual cells and RNAscope smFISH has validated heterogeneity at the mRNA level and facilitated spatial localization of cell subsets.
Miguel Medina
Miguel Medina, Ph.D.
Account Executive
Bio-Techne

Miguel Medina is an Account Executive with Advanced Cell Diagnostics, a Bio-Techne brand. He received in Ph.D. in Immunology from Baylor College of Medicine and trained as a post-doc at MD Anderson developing cancer vaccine in the department of Melanoma before joining biotechnology sales. He has worked with various companies focusing in the areas of immunology, diagnostics, and gene sequencing. From his days in the lab and presently, he enjoys employing new scientific technologies that help answer scientific questions for humankind.