Flow cytometry is a fluorescence-based technology that allows multiple targets to be analyzed simultaneously. However, the number of targets is limited by the cytometer at your disposal i.e. the lasers and optical filters inside. As the number of fluorochromes available has increased in recent years, knowledge of the pros and cons of each is vital in order to build an effective multicolor flow cytometry panel. Allied to this is knowing the optical layout of the cytometer you are using. In this webinar, I will highlight important considerations, and give tips on how to effectively and efficiently use a flow cytometer for multicolor phenotyping experiments.
During the webinar, viewers will:
- Learn the basic principles behind flow panel design
- Understand the pros and cons of different fluorochromes
- Get tips on how to effectively use a flow cytometer for multicolor experiments
- Have the opportunity to ask questions during the live broadcast
SPEAKER
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Derek Davies Science Technology Platform Training Lead Flow Cytometry The Francis Crick Institute
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Derek Davies has been active in flow cytometry for over 30 years. He was head of the flow cytometry core facility at the Cancer Research UK London Research Institute, and then the facility at the Francis Crick Institute in London, UK. Derek has taught on several flow cytometry courses in the UK and beyond. He has been a chair of the flow cytometry section of the Royal Microscopical Society, chair of flowcytometryUK and served as an ISAC councillor. Recently, he has moved into a training role which allows him to use his years of experience in the field to help guide newcomers through the sometimes challenging world of flow cytometry.
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Jody Bonnevier, Ph.D. Antibody Development Manager Bio-Techne
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Dr. Jody Bonnevier is a Manager in the Antibody Development Department at R&D Systems in Minneapolis MN. Jody received her PhD in Microbiology, Immunology, and Cancer Biology at the University of Minnesota, and has over 10 years of experience developing novel antibodies and reagents, with a focus on flow cytometry and cell selection product development. |