Coronavirus disease 2019 (COVID-19) is caused by a novel coronavirus, SARS-CoV-2. Its emergence was identified in late December 2019 from Wuhan, Hubei province China. As of Spring 2021, over 167 million cases and over 3 million deaths have been reported globally. Initial reports centered around a potentially fatal atypical pneumonia. As cases and ensuing research progressed, ACE-2 was identified as the cellular receptor for the SARS-CoV-2 virus, primarily through interaction between the receptor binding domain (RBD) on the viral surface spike protein. The most commonly affected systems are the upper and lower respiratory, cardiovascular, renal, and neural. Patients have presented a broad category of symptoms associated with infection, while some remain asymptomatic. While a global immunization effort has reduced overall cases and deaths, research continues into the etiology of infection due to SARS-CoV-2, variants and long-term complications from SARS-CoV-2.
In this webinar, you will learn how to complement your current Immunohistochemistry (IHC) protein detection workflow with RNA ISH co-detection using RNAscope®. ACD, a Bio-Techne brand, has developed a new RNA-Protein Co-detection Kit that allows compatibility with IHC antibodies for simultaneous detection of proteins and RNA.
We will examine how this new assay and workflow preserves precious autopsy samples from SARS-CoV-2 infected tissues and augments ISH and IHC data. This allows greater understanding of cellular mechanisms underlying SARS-CoV-2 infection and transmission.
The co-detection assay may be performed either manually or using Leica platforms. We will discuss the technique while reviewing results on SARS-CoV-2 infected tissues.
SPEAKER
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Alex Kalyuzhny, PhD.
Scientific Manager of IHC and ELISpot
Bio-Techne
[email protected]
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Dr. Alex Kalyuzhny is a Scientific Manager of Immunohistochemistry and the ELISpot laboratory at Bio-Techne in Minneapolis, MN. Alex received his PhD in Embryology and Histology at the Institute of Development Biology, Russian Academy of Sciences. He has over 20 years of experience developing novel antibodies and ELISpot assays for research and clinical applications.
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Steve Orstad
Senior Product Manager
Bio-Techne
[email protected]
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Steve Orstad is the Senior Product Manager for R&D Systems brand antibodies at Bio-Techne. Steve graduated from Concordia College-Moorhead, MN with a Bachelor of Arts in Biology. He has held several positions in Technical Service and Marketing since starting at Bio-Techne in 2001.
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