Join us as we discuss how this modified protocol on the plant leaf tissues can be adapted easily on other plant species and provides a powerful tool of RNAscope for RNA visualization.
RNAscope multiplex fluorescent RNA-ISH detection is a powerful and sensitive tool for spatio-temporal RNA species detection in tissues. However, due to plant tissues' morphological and structural characteristics, its implication was not reported until Solanki et al., 2020 (https://doi.org/10.1186/s13007-020-00614-4) presented a modified protocol of tissue preparation and probe hybridization. This modified protocol on the barley leaf tissues can be adapted easily on other plant species and provides a powerful tool for plant researchers and cell biologists.
In this webinar, we will discuss a practical approach of carrying out RNAscope assay using a parallel comparison of plant and animal samples to avoid common pitfalls and increase the chances of a successful experiment.
LEARNING OBJECTIVES
- Sample visualization and image analysis
SPEAKERS
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Shyam Solanki, Ph.D. Postdoctoral Research Associate,
Dept. of Crop and Soil Sciences Washington State University
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Jyoti Phatak-Sheldon, M.S. Associate Scientist, Applications,
Advanced Cell Diagnostics, a Bio-Techne brand
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